TB-500 (Thymosin Beta-4 Fragment) Dosage Protocols: All Vial Sizes
TB-500 is a synthetic fragment of Thymosin Beta-4, a regenerative peptide found in nearly all human cell types. It is studied for tissue repair, wound healing, and cardiac regeneration. Choose your vial size below; each size has its own exact dosing table.
Choose Your Vial Size
| Vial | Final volume | Concentration | One U-100 unit | |
|---|---|---|---|---|
| 5 mg | 3 mL | 1.67 mg/mL | 16.67 mcg in 0.01 mL | Open protocol ā |
| 10 mg | 3 mL | 3.33 mg/mL | 33.33 mcg in 0.01 mL | Open protocol ā |
| 20 mg | 3 mL | 6.67 mg/mL | 66.67 mcg in 0.01 mL | Open protocol ā |
Doses are the same research amounts across sizes; the vial size changes the concentration, so syringe units differ. Always use the table for your exact vial.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | ā20Ā°C (ā4Ā°F) | Up to 24 months, dry & dark |
| Reconstituted | 2ā8Ā°C (35ā46Ā°F) | Up to 30 days, protect from light |
Important Notes
How This Works
TB-500 is a synthetic fragment of Thymosin Beta-4 (TĪ²4), a 43-amino-acid protein found in virtually every human cell and concentrated in platelets and wound fluid. The active TB-500 region binds G-actin and regulates cytoskeletal organization and cell motility.
It promotes endothelial cell migration, accelerates wound closure, supports cardiomyocyte recovery after infarction, reduces fibrosis, and modulates inflammatory cytokine release. The peptide also up-regulates laminin-5 and integrin-linked kinase, both involved in cell-matrix adhesion during repair.
Potential Benefits & Side Effects
Potential Benefits
- Accelerated wound closure and reduced scarring in rodent models.
- Cardioprotective effects post-infarction in mouse models.
- Promotes hair regrowth in animal alopecia models.
- Anti-inflammatory action via NF-ĪŗB downregulation.
- Synergy with BPC-157 in connective-tissue protocols.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site soreness possible.
- Transient fatigue during first 1ā2 weeks of loading.
- Theoretical pro-angiogenic concerns with occult malignancy.
Lifestyle Factors
- Pair with physical therapy for tendon/ligament work.
- Adequate protein (1.2ā1.6 g/kg/day).
- Vitamin C, zinc, copper sufficiency support wound healing.
- Avoid chronic NSAIDs during active connective-tissue repair.
Injection Technique
- Subcutaneous ā abdomen, thigh, or near the injury site.
- Rotate sites with each dose during loading phase.
- 90Ā° angle with short insulin needle.