TB-500 (Thymosin Beta-4 Fragment) (20 mg Vial) Dosage Protocol
TB-500 is a synthetic fragment of Thymosin Beta-4, a regenerative peptide found in nearly all human cell types. It is studied for tissue repair, wound healing, and cardiac regeneration. This page covers the 20 mg vial.
ā” Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard / Gradual Approach (3 mL = 6.67 mg/mL)
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1ā2 | 500 mcg | 7.5 units (0.075 mL) |
| Weeks 3ā4 | 600 mcg | 9 units (0.09 mL) |
| Weeks 5ā8 | 750 mcg | 11.25 units (0.113 mL) |
| Weeks 9ā12 | 1000 mcg | 15 units (0.15 mL) |
Frequency shown: Once daily by subcutaneous injection. Community-reported use: The table preserves a recurring staged pattern for calculation and comparison. It is not a recommendation and is not supported by a human TB-500 dose-ranging trial.
Reconstitution Steps
- Draw 3 mL bacteriostatic water with a sterile syringe.
- Inject the diluent slowly down the inside wall of the vial.
- Gently swirl until dissolved. Do not shake.
- Label with the compound, concentration, and preparation date; refrigerate at 2-8 Ā°C (36-46 Ā°F) and protect from light.
Research reference only: The staged schedule is a calculation example drawn from community descriptions. The cited TB-500 analytical studies, animal experiments, and thymosin beta-4 trials do not establish these amounts, this route, or this duration as a safe or effective human regimen.
Supplies Needed
Calculated from the schedule above for a 20 mg vial reconstituted with 3 mL (one injection per day, two swabs per injection).
| Item | 8 weeks | 12 weeks | 16 weeks |
|---|---|---|---|
| Peptide vials (20 mg each) | 2 vials (36.4 mg total) | 4 vials (64.4 mg total) | 5 vials (92.4 mg total) |
| Insulin syringes (U-100) | 56 syringes | 84 syringes | 112 syringes |
| Bacteriostatic water (10 mL bottles) | 6 mL ā 1 Ć 10 mL bottle | 12 mL ā 2 Ć 10 mL bottles | 15 mL ā 2 Ć 10 mL bottles |
| Alcohol swabs | 112 swabs ā 2 Ć 100-count boxes | 168 swabs ā 2 Ć 100-count boxes | 224 swabs ā 3 Ć 100-count boxes |
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | ā20Ā°C (ā4Ā°F) | Up to 24 months, dry & dark |
| Reconstituted | 2ā8Ā°C (35ā46Ā°F) | Up to 30 days, protect from light |
Important Notes
How This Works
TB-500 is a synthetic fragment of Thymosin Beta-4 (TĪ²4), a 43-amino-acid protein found in virtually every human cell and concentrated in platelets and wound fluid. The active TB-500 region binds G-actin and regulates cytoskeletal organization and cell motility.
It promotes endothelial cell migration, accelerates wound closure, supports cardiomyocyte recovery after infarction, reduces fibrosis, and modulates inflammatory cytokine release. The peptide also up-regulates laminin-5 and integrin-linked kinase, both involved in cell-matrix adhesion during repair.
Potential Benefits & Side Effects
Potential Benefits
- Accelerated wound closure and reduced scarring in rodent models.
- Cardioprotective effects post-infarction in mouse models.
- Promotes hair regrowth in animal alopecia models.
- Anti-inflammatory action via NF-ĪŗB downregulation.
- Synergy with BPC-157 in connective-tissue protocols.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site soreness possible.
- Transient fatigue during first 1ā2 weeks of loading.
- Theoretical pro-angiogenic concerns with occult malignancy.
Lifestyle Factors
- Pair with physical therapy for tendon/ligament work.
- Adequate protein (1.2ā1.6 g/kg/day).
- Vitamin C, zinc, copper sufficiency support wound healing.
- Avoid chronic NSAIDs during active connective-tissue repair.
Injection Technique
- Subcutaneous ā abdomen, thigh, or near the injury site.
- Rotate sites with each dose during loading phase.
- 90Ā° angle with short insulin needle.