TB-500 (Thymosin Beta-4 Fragment) (5 mg Vial) Dosage Protocol
TB-500 is a synthetic fragment of Thymosin Beta-4, a regenerative peptide found in nearly all human cell types. It is studied for tissue repair, wound healing, and cardiac regeneration. This page covers the 5 mg vial.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard / Gradual Approach (3 mL = ~1.67 mg/mL)
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 500 mcg | 30 units (0.30 mL) |
| Weeks 3–4 | 600 mcg | 36 units (0.36 mL) |
| Weeks 5–8 | 750 mcg | 45 units (0.45 mL) |
| Weeks 9–12 | 1000 mcg | 60 units (0.60 mL) |
Frequency: Inject once daily subcutaneously. This schedule uses the largest practical dilution (3.0 mL) to keep per‑injection units ≥30 for excellent accuracy. Total weekly dose averages ~5 mg. These amounts remain the page’s established calculation schedule and are not a regimen established by human TB-500 fragment research.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label with date and concentration; refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. TB‑500 is not FDA‑approved for human use and is for research purposes only.
Supplies Needed
Plan based on an 8–16 week daily protocol with gradual titration.
| Item | Quantity |
|---|---|
| Peptide Vials (TB‑500, 5 mg each) | 8 weeks: approximately 8 vials required 12 weeks: approximately 12 vials required 16 weeks: approximately 16 vials required |
| Insulin Syringes (U‑100) | Per week: 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes 16 weeks: 112 syringes |
| Bacteriostatic Water (10 mL bottles) | 8 weeks (8 vials): 24 mL — 3 bottles required (10 mL each) 12 weeks (12 vials): 36 mL — 4 bottles required (10 mL each) 16 weeks (16 vials): 48 mL — 5 bottles required (10 mL each) |
| Alcohol Swabs | Per week: 14 swabs (2/day) 8 weeks: 112 swabs — 2 boxes required (100 swabs each) 12 weeks: 168 swabs — 2 boxes required (100 swabs each) 16 weeks: 224 swabs — 3 boxes required (100 swabs each) |
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
TB-500 is a synthetic fragment of Thymosin Beta-4 (Tβ4), a 43-amino-acid protein found in virtually every human cell and concentrated in platelets and wound fluid. The active TB-500 region binds G-actin and regulates cytoskeletal organization and cell motility.
It promotes endothelial cell migration, accelerates wound closure, supports cardiomyocyte recovery after infarction, reduces fibrosis, and modulates inflammatory cytokine release. The peptide also up-regulates laminin-5 and integrin-linked kinase, both involved in cell-matrix adhesion during repair.
Potential Benefits & Side Effects
Potential Benefits
- Accelerated wound closure and reduced scarring in rodent models.
- Cardioprotective effects post-infarction in mouse models.
- Promotes hair regrowth in animal alopecia models.
- Anti-inflammatory action via NF-κB downregulation.
- Synergy with BPC-157 in connective-tissue protocols.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site soreness possible.
- Transient fatigue during first 1–2 weeks of loading.
- Theoretical pro-angiogenic concerns with occult malignancy.
Lifestyle Factors
- Pair with physical therapy for tendon/ligament work.
- Adequate protein (1.2–1.6 g/kg/day).
- Vitamin C, zinc, copper sufficiency support wound healing.
- Avoid chronic NSAIDs during active connective-tissue repair.
Injection Technique
- Subcutaneous — abdomen, thigh, or near the injury site.
- Rotate sites with each dose during loading phase.
- 90° angle with short insulin needle.