SS-31 (Elamipretide) (10 mg Vial) Dosage Protocol
SS-31 (elamipretide) is a mitochondrial-targeted tetrapeptide that binds cardiolipin in the inner mitochondrial membrane, stabilizing electron-transport chain function. This page covers the 10 mg vial.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard / Gradual Approach (1 mL = 10 mg/mL)
| Week | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 5 mg (5000 mcg) | 50 units (0.50 mL) |
| Weeks 3–8 | 10 mg (10,000 mcg) | 100 units (1.0 mL) |
Frequency: Inject once daily subcutaneously at a consistent time. This concentrated dilution (1.0 mL per 10 mg vial) keeps standard doses within a single insulin syringe for convenience and accuracy.
Reconstitution Steps
- Draw 1.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid vigorous shaking to prevent foaming.
- Gently swirl/roll until fully dissolved (clear solution).
- Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; follow product-specific use-by and sterile-handling requirements.
Advanced / Aggressive Approach (1 mL = 10 mg/mL)
| Week | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 5 mg (5000 mcg) | 50 units (0.50 mL) |
| Weeks 3–4 | 10 mg (10,000 mcg) | 100 units (1.0 mL) |
| Weeks 5–8 | 15 mg (15,000 mcg) | Split: 2 × 75 units (0.75 mL each) |
| Optional Weeks 9–12 | 20 mg (20,000 mcg) | Split: 2 × 100 units (1.0 mL each) |
Note: Advanced dosing (15–20 mg/day) is based on short‑term clinical trial protocols for severe mitochondrial conditions and should only be pursued under medical supervision. Doses above 10 mg require splitting into two separate subcutaneous injections at different sites. Clinical trials have not extensively evaluated SS‑31 beyond 12 weeks; extended use requires careful monitoring.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Plan based on an 8–12 week daily protocol with gradual titration.
| Item | Quantity |
|---|---|
| Peptide Vials (SS‑31, 10 mg each) | 8 weeks (Standard: 5–10 mg/day): approximately 50 vials required 12 weeks (Standard maintenance at 10 mg/day): approximately 77 vials required 12 weeks (Advanced: escalating to 15 mg/day): approximately 105 vials required |
| Insulin Syringes (U‑100, 1 mL capacity) | Per week (standard dosing): 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes Advanced protocols with split injections may require 2 syringes per day for higher doses |
| Bacteriostatic Water (30 mL bottles) | 8 weeks (50 vials): 50 mL — 2 bottles required (30 mL each) 12 weeks (77 vials): 77 mL — 3 bottles required (30 mL each) 12 weeks (105 vials, advanced): 105 mL — 4 bottles required (30 mL each) |
| Alcohol Swabs | Per week: 14 swabs (2/day) 8 weeks: 112 swabs — 2 boxes required (100 swabs each) 12 weeks: 168 swabs — 2 boxes required (100 swabs each) |
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
SS-31 is a synthetic tetrapeptide (D-Arg-2',6'-dimethylTyr-Lys-Phe-NH2) of the Szeto-Schiller class. Its alternating cationic-aromatic structure concentrates it in the inner mitochondrial membrane (1000–5000× more than cytoplasm). It binds cardiolipin — the unique phospholipid that organizes the electron-transport chain — preserving cristae structure and ATP synthesis under oxidative stress.
Potential Benefits & Side Effects
Potential Benefits
- Selective mitochondrial targeting via cardiolipin binding.
- Stabilization of electron-transport chain efficiency.
- Preservation of ATP synthesis under oxidative stress.
- Studied in mitochondrial myopathy.
- Cardioprotective effects in ischemia-reperfusion.
Side Effect Profile
- Generally well-tolerated.
- Injection-site reactions most common.
- Mild transient nausea or fatigue.
Lifestyle Factors
- Pair with aerobic exercise to amplify mitochondrial biogenesis.
- Adequate sleep supports mitochondrial recovery.
- CoQ10, alpha-lipoic acid sufficiency complement effects.
Injection Technique
- Subcutaneous, abdomen/thigh/upper arm. Rotate sites.
- 90° angle with short insulin needle.