SS-31 (Elamipretide) Dosage Protocols: All Vial Sizes
SS-31 (elamipretide) is a mitochondrial-targeted tetrapeptide that binds cardiolipin in the inner mitochondrial membrane, stabilizing electron-transport chain function. Choose your vial size below; each size has its own exact dosing table.
Choose Your Vial Size
| Vial | Final volume | Concentration | One U-100 unit | |
|---|---|---|---|---|
| 10 mg | 1 mL | 10 mg/mL | 100 mcg in 0.01 mL | Open protocol → |
| 50 mg | 3 mL | 16.67 mg/mL | 166.67 mcg in 0.01 mL | Open protocol → |
Doses are the same research amounts across sizes; the vial size changes the concentration, so syringe units differ. Always use the table for your exact vial.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
SS-31 is a synthetic tetrapeptide (D-Arg-2',6'-dimethylTyr-Lys-Phe-NH2) of the Szeto-Schiller class. Its alternating cationic-aromatic structure concentrates it in the inner mitochondrial membrane (1000–5000× more than cytoplasm). It binds cardiolipin — the unique phospholipid that organizes the electron-transport chain — preserving cristae structure and ATP synthesis under oxidative stress.
Potential Benefits & Side Effects
Potential Benefits
- Selective mitochondrial targeting via cardiolipin binding.
- Stabilization of electron-transport chain efficiency.
- Preservation of ATP synthesis under oxidative stress.
- Studied in mitochondrial myopathy.
- Cardioprotective effects in ischemia-reperfusion.
Side Effect Profile
- Generally well-tolerated.
- Injection-site reactions most common.
- Mild transient nausea or fatigue.
Lifestyle Factors
- Pair with aerobic exercise to amplify mitochondrial biogenesis.
- Adequate sleep supports mitochondrial recovery.
- CoQ10, alpha-lipoic acid sufficiency complement effects.
Injection Technique
- Subcutaneous, abdomen/thigh/upper arm. Rotate sites.
- 90° angle with short insulin needle.