Glutathione (1500 mg Vial) Dosage Protocol
Glutathione is a tripeptide (Glu-Cys-Gly) and the body's primary intracellular antioxidant. Used in IV nutrient therapy and research for oxidative stress modulation.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard / Gradual Approach (3 mL = 500 mg/mL)
| Week | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 100 mg | 20 units (0.2 mL) |
| Weeks 3–4 | 150 mg | 30 units (0.3 mL) |
| Weeks 5–8 | 200 mg | 40 units (0.4 mL) |
Frequency: Inject once daily subcutaneously, or split into every-other-day dosing based on protocol goals. Some protocols utilize 200 mg 1–2 × weekly for maintenance. This schedule uses a practical 2.0 mL dilution for accurate measurements with standard insulin syringes.
Reconstitution Steps
- Draw 3 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Calculated from the schedule above for a 1500 mg vial reconstituted with 3 mL (one injection per day, two swabs per injection).
| Item | 8 weeks | 12 weeks | 16 weeks |
|---|---|---|---|
| Peptide vials (1500 mg each) | 7 vials (9100 mg total) | 10 vials (14700 mg total) | 14 vials (20300 mg total) |
| Insulin syringes (U-100) | 56 syringes | 84 syringes | 112 syringes |
| Bacteriostatic water (10 mL bottles) | 21 mL → 3 × 10 mL bottles | 30 mL → 3 × 10 mL bottles | 42 mL → 5 × 10 mL bottles |
| Alcohol swabs | 112 swabs → 2 × 100-count boxes | 168 swabs → 2 × 100-count boxes | 224 swabs → 3 × 100-count boxes |
Protocol Overview
The peptide replenishes intracellular antioxidant pools depleted by oxidative stress, alcohol metabolism, and aging.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 21 days, protect from light |
Important Notes
How This Works
Glutathione is a tripeptide of glutamate, cysteine, and glycine — the primary intracellular antioxidant in mammals. It directly neutralizes reactive oxygen species, regenerates other antioxidants (vitamin C, E), supports phase-2 hepatic detoxification, and modulates immune cell function.
Potential Benefits & Side Effects
Potential Benefits
- Replenishes glutathione pools depleted by oxidative stress.
- Supports hepatic detoxification.
- Used in IV nutrient therapy practice.
- Antioxidant effects in cellular and clinical models.
Side Effect Profile
- Generally well-tolerated.
- Sulfur-rich smell sometimes noticeable.
- Mild injection-site reactions.
Lifestyle Factors
- Adequate sleep, vitamin C, and N-acetylcysteine support endogenous synthesis.
- Limit alcohol — alcohol metabolism depletes glutathione.
Injection Technique
- Intramuscular (gluteal, deltoid) or slow IV in clinical settings.