Glutathione (1500 mg Vial) Dosage Protocol
Glutathione is a tripeptide (Glu-Cys-Gly) and the body's primary intracellular antioxidant. Used in IV nutrient therapy and research for oxidative stress modulation.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous (or slow IV in clinical settings) | Frequency: Once daily | Half-life: ~10 minutes (cellular pool replenishment lasts longer)
Standard Approach (5 mL = 300.00 mg/mL)
Reconstituting with 5 mL bacteriostatic water produces a concentration of 300.00 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Weeks 1–2 (titration) | 100 mg | 33.3 units | 0.33 mL | 15 doses |
| Weeks 3–4 | 150 mg | 50 units | 0.50 mL | 10 doses |
| Weeks 5–8 (target) | 200 mg | 66.7 units | 0.67 mL | 7 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 5 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 21 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 150 mg per dose, once daily (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Glutathione (1500 mg) vials | 6 vials | 9 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 3 × 10 mL | 5 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Glutathione protocols typically run 1–2× weekly intramuscular or weekly slow IV (clinical setting) over 8–12 week cycles. The peptide replenishes intracellular antioxidant pools depleted by oxidative stress, alcohol metabolism, and aging.
Dosing Protocol
Daily titration (subcutaneous):
- Weeks 1–2 (titration): 100 mg/day.
- Weeks 3–4: 150 mg/day.
- Weeks 5–8 (target): 200 mg/day.
IV nutrient-clinic protocols: 600–1500 mg slow IV/IM, 1–2× weekly. Clinical setting required; do not attempt at home.
Cycle structure: 8–12 weeks daily, then reassessment. Effects on oxidative-stress markers accumulate over the cycle.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 21 days, protect from light |
Important Notes
How This Works
Glutathione is a tripeptide of glutamate, cysteine, and glycine — the primary intracellular antioxidant in mammals. It directly neutralizes reactive oxygen species, regenerates other antioxidants (vitamin C, E), supports phase-2 hepatic detoxification, and modulates immune cell function.
Potential Benefits & Side Effects
Potential Benefits
- Replenishes glutathione pools depleted by oxidative stress.
- Supports hepatic detoxification.
- Used in IV nutrient therapy practice.
- Antioxidant effects in cellular and clinical models.
Side Effect Profile
- Generally well-tolerated.
- Sulfur-rich smell sometimes noticeable.
- Mild injection-site reactions.
Lifestyle Factors
- Adequate sleep, vitamin C, and N-acetylcysteine support endogenous synthesis.
- Limit alcohol — alcohol metabolism depletes glutathione.
Injection Technique
- Intramuscular (gluteal, deltoid) or slow IV in clinical settings.