GHK-Cu (Copper Peptide) Dosage Protocols: All Vial Sizes
GHK-Cu is a copper-binding tripeptide (glycyl-L-histidyl-L-lysine) studied for skin remodeling, wound healing, and hair growth. Native plasma levels decline with age in parallel with regenerative capacity. Choose your vial size below; each size has its own exact dosing table.
Choose Your Vial Size
| Vial | Final volume | Concentration | One U-100 unit | |
|---|---|---|---|---|
| 50 mg | 3 mL | 16.67 mg/mL | 166.67 mcg in 0.01 mL | Open protocol → |
| 100 mg | 3 mL | 33.33 mg/mL | 333.33 mcg in 0.01 mL | Open protocol → |
Doses are the same research amounts across sizes; the vial size changes the concentration, so syringe units differ. Always use the table for your exact vial.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
GHK is a tripeptide (Gly-His-Lys) discovered in human plasma in 1973. Its bioactive form binds copper(II) to form GHK-Cu. Plasma levels decline from ~200 ng/mL in young adults to ~80 ng/mL by age 60, paralleling tissue regenerative decline.
GHK-Cu modulates over 4,000 human genes — stimulating collagen, elastin, and proteoglycan synthesis; activating Nrf2 antioxidant defense; upregulating stem-cell factor and dermal hair-follicle stem-cell activity; downregulating pro-inflammatory cytokines.
Potential Benefits & Side Effects
Potential Benefits
- Increased dermal collagen and elastin synthesis.
- Reduction of fine lines and improved skin firmness.
- Accelerated wound and ulcer healing.
- Hair-growth stimulation in animal alopecia models.
- Antioxidant and anti-inflammatory via Nrf2.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site irritation possible.
- Topical use may cause mild redness/itching.
- Theoretical concern with copper-overload disorders (Wilson disease).
Lifestyle Factors
- Pair with adequate vitamin C and zinc for collagen synthesis.
- Sun protection preserves skin gains.
- Hydration supports dermal function.
- Topical applications work well alongside subcutaneous protocols.
Injection Technique
- Subcutaneous into abdomen, thigh, or near area of interest.
- Rotate injection sites with each dose.
- 90° angle with short insulin needle.
- Topical: apply to clean dry skin; allow to absorb.