Epitalon (50 mg Vial) Dosage Protocol
Epitalon is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) developed from the pineal gland peptide Epithalamin. Russian research groups have studied it for telomere maintenance and longevity outcomes. This page covers the 50 mg vial.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard Protocol (2 mL = 25 mg/mL)
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Days 1–20 (Cycle On) | 5,000 mcg (5 mg) | 20 units (0.2 mL) |
| Weeks 4–26 (Cycle Off) | 0 mcg | — |
Frequency: Inject once daily subcutaneously, preferably in the evening or at bedtime to synergize with natural melatonin rhythms. Complete 20 consecutive days, then observe a 4–6 month off-cycle before repeating (typically 2 cycles per year).
Reconstitution Steps
- Draw 2 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Calculated from the schedule above for a 50 mg vial reconstituted with 2 mL (one injection per day, two swabs per injection).
| Item | One 20-day cycle |
|---|---|
| Peptide vials (50 mg each) | 2 vials (100 mg total) |
| Insulin syringes (U-100) | 20 syringes |
| Bacteriostatic water (10 mL bottles) | 4 mL → 1 × 10 mL bottle |
| Alcohol swabs | 40 swabs → 1 × 100-count box |
Protocol Overview
Effects on melatonin secretion patterns and circadian rhythm reportedly persist months past the cycle's end, supporting the cycle-based rather than continuous approach.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
Epitalon is a synthetic tetrapeptide derived from Epithalamin, originally identified by Vladimir Khavinson's group. Reported actions include telomerase upregulation in human somatic cell culture, restoration of pineal melatonin secretion toward youthful patterns, normalization of disrupted circadian cortisol rhythms, and gene-expression modulation in immune and neuroendocrine tissues.
Potential Benefits & Side Effects
Potential Benefits
- Reported telomerase upregulation in cell culture.
- Restoration of melatonin secretion patterns in elderly.
- Normalization of disrupted circadian rhythms.
- Russian observational studies report mortality reduction in elderly cohorts.
- Antioxidant effects via gene-expression modulation.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site reactions.
- Vivid dreams reported.
Lifestyle Factors
- Cycle-based dosing — typical 10–20 day cycle, 1–2× per year.
- Evening dosing aligns with pineal/melatonin axis.
- Pair with circadian-aligned light exposure.
Injection Technique
- Subcutaneous, rotate sites if dosing daily during cycle.