DSIP (Delta Sleep-Inducing Peptide) (15 mg Vial) Dosage Protocol
DSIP is a 9-amino-acid neuropeptide first isolated from rabbit cerebral venous blood during electrically induced sleep. It modulates delta-wave sleep and exhibits anti-stress effects. This page covers the 15 mg vial.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Pre-bedtime | Half-life: ~7 minutes (extended CNS effect)
Standard Approach (3 mL = 5.00 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 5.00 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Week 1 (titration) | 100 mcg | 2 units | 0.020 mL | 150 doses |
| Week 2 | 150 mcg | 3 units | 0.030 mL | 100 doses |
| Week 3 | 200 mcg | 4 units | 0.040 mL | 75 doses |
| Weeks 4–8 (target) | 275 mcg | 5.5 units | 0.055 mL | 54 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 30 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 200 mcg per dose, pre-bedtime (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| DSIP (Delta Sleep-Inducing Peptide) (15 mg) vials | 1 vials | 2 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 1 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
DSIP is dosed pre-bedtime nightly during a cycle. Effects compound — most users report sleep-quality improvement after 1–2 weeks of consistent use rather than acute changes.
Cycles typically run 2–4 weeks for situational insomnia or stress-recovery protocols.
Dosing Protocol
Pre-bedtime dosing:
- Starting (100 mcg): Conservative starting dose.
- Standard (200 mcg): Common research dose.
- Higher (300 mcg): Persistent insomnia protocols.
Cycle structure: 2–4 weeks active, then break. Continuous nightly use is sometimes done; the literature supports cycled approaches better.
Timing: 30–60 min before bedtime in a calm setting. Avoid screens, caffeine, alcohol within 2h of dosing.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
DSIP (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) was first isolated from venous blood of rabbits during electrically induced sleep. Despite its name it does not directly induce sleep but appears to modulate sleep architecture, particularly delta-wave activity. It modulates the HPA axis with cortisol-lowering effects under stress, alters limbic neurotransmitter release, and exerts antioxidant and anti-convulsant activity.
Potential Benefits & Side Effects
Potential Benefits
- Modulation of slow-wave sleep architecture.
- Anti-stress via HPA axis modulation — reduced cortisol.
- Anxiolytic and mild analgesic in animal models.
- Antioxidant effects on brain tissue.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site discomfort.
- Vivid dreams reported.
- Daytime drowsiness if dosed too late.
Lifestyle Factors
- Inject 30–60 minutes before bedtime in a calm setting.
- Consistent sleep-wake schedule for best results.
- Avoid screens, caffeine, alcohol within 2 hours of dosing.
- Effects accumulate — many users report results after 1–2 weeks.
Injection Technique
- Subcutaneous, rotate sites if dosing nightly.
- Pre-bedtime timing is universal — DSIP is short-acting.