DSIP (Delta Sleep-Inducing Peptide) (15 mg Vial) Dosage Protocol
DSIP is a 9-amino-acid neuropeptide first isolated from rabbit cerebral venous blood during electrically induced sleep. It modulates delta-wave sleep and exhibits anti-stress effects. This page covers the 15 mg vial.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and nightly dosing
Standard / Gradual Approach (3 mL = 5 mg/mL)
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Week 1 | 100 mcg | 2 units (0.02 mL) |
| Week 2 | 150 mcg | 3 units (0.03 mL) |
| Week 3 | 200 mcg | 4 units (0.04 mL) |
| Weeks 4–8 | 250–300 mcg | 5–6 units (0.05–0.06 mL) |
Frequency: Inject once daily subcutaneously in the evening, approximately 30–60 minutes before bedtime. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.
Reconstitution Steps
- Draw 3 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Calculated from the schedule above for a 15 mg vial reconstituted with 3 mL (one injection per day, two swabs per injection).
| Item | 8 weeks | 12 weeks | 16 weeks |
|---|---|---|---|
| Peptide vials (15 mg each) | 1 vial (13.65 mg total) | 2 vials (22.05 mg total) | 3 vials (30.45 mg total) |
| Insulin syringes (U-100) | 56 syringes | 84 syringes | 112 syringes |
| Bacteriostatic water (10 mL bottles) | 3 mL → 1 × 10 mL bottle | 6 mL → 1 × 10 mL bottle | 9 mL → 1 × 10 mL bottle |
| Alcohol swabs | 112 swabs → 2 × 100-count boxes | 168 swabs → 2 × 100-count boxes | 224 swabs → 3 × 100-count boxes |
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
DSIP (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) was first isolated from venous blood of rabbits during electrically induced sleep. Despite its name it does not directly induce sleep but appears to modulate sleep architecture, particularly delta-wave activity. It modulates the HPA axis with cortisol-lowering effects under stress, alters limbic neurotransmitter release, and exerts antioxidant and anti-convulsant activity.
Potential Benefits & Side Effects
Potential Benefits
- Modulation of slow-wave sleep architecture.
- Anti-stress via HPA axis modulation — reduced cortisol.
- Anxiolytic and mild analgesic in animal models.
- Antioxidant effects on brain tissue.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site discomfort.
- Vivid dreams reported.
- Daytime drowsiness if dosed too late.
Lifestyle Factors
- Inject 30–60 minutes before bedtime in a calm setting.
- Consistent sleep-wake schedule for best results.
- Avoid screens, caffeine, alcohol within 2 hours of dosing.
- Effects accumulate — many users report results after 1–2 weeks.
Injection Technique
- Subcutaneous, rotate sites if dosing nightly.
- Pre-bedtime timing is universal — DSIP is short-acting.