Research use only.  Educational reference. Not medical advice. Not for human consumption.
💊 VITAMIN / NUTRITIONAL

Vitamin B12 (Cyanocobalamin) Injectable

Injectable B12 (cyanocobalamin) delivers cobalamin directly into circulation, bypassing GI absorption barriers that limit oral supplementation. Essential for neurological function, red blood cell synthesis, and methylation reactions. Commonly included in lipotropic injection programs.

⚡ QUICKSTART HIGHLIGHTS

CONCENTRATION
1,000 mcg/mL (standard)
TYPICAL DOSE
1,000 mcg per injection
1 U-100 UNIT =
10 mcg
FREQUENCY
Weekly (maintenance) or daily × 7 days (loading)

Dosing & Reconstitution Guide

Vial SizeBAC WaterConcentrationmcg per Unit
1 mg (1,000 mcg)1.0 mL1,000 mcg/mL10 mcg
5 mg (5,000 mcg)5.0 mL1,000 mcg/mL10 mcg

Dosing Protocols

ProtocolDoseU-100 UnitsVolumeFrequency
Maintenance1,000 mcg100 units1.0 mLOnce weekly or monthly
Loading (deficiency)1,000 mcg100 units1.0 mLDaily × 7 days, then monthly
With MIC/Lipo injection1,000 mcg (included in blend)Weekly or 2× weekly

Reconstitution Steps

  1. Wipe the vial stopper and BAC water vial with alcohol; let dry.
  2. Draw 1.0 (or 5.0 for 5mg vial) mL of bacteriostatic water into a sterile syringe.
  3. Inject slowly down the inside glass wall of the B12 vial. Do not aim at the powder.
  4. Gently swirl until fully dissolved. Do not shake.
  5. Label with reconstitution date. Refrigerate at 2–8°C; use within the window specified above.
Note: B12 is light-sensitive. Store reconstituted solution protected from light. The standard concentration is 1,000 mcg/mL; always verify the label on your specific vial.

How This Works

Cyanocobalamin is the most stable and widely used synthetic form of vitamin B12. After injection it is converted in the liver to methylcobalamin and adenosylcobalamin — the two biologically active coenzyme forms. Methylcobalamin is essential for methionine synthase (converting homocysteine to methionine, critical for DNA methylation) and adenosylcobalamin is required for methylmalonyl-CoA mutase (fatty acid and amino acid catabolism).

B12 deficiency is common in vegans/vegetarians, elderly individuals (reduced intrinsic factor), patients taking metformin (which blocks B12 absorption), and those with GI disorders. Deficiency causes macrocytic anemia, subacute combined degeneration of the spinal cord, and cognitive impairment. Injectable B12 achieves near-100% bioavailability regardless of intrinsic factor status.

Potential Benefits & Side Effects

Potential Benefits

  • Corrects B12 deficiency with near-100% bioavailability (vs 1-2% for high-dose oral in pernicious anemia).
  • Supports neurological function — myelin synthesis and nerve conduction.
  • Required for red blood cell maturation — prevents megaloblastic anemia.
  • Lowers homocysteine via methionine cycle support.
  • Energy metabolism: cofactor in mitochondrial reactions.
  • Frequently combined with lipotropic injections (MIC, Lipo-B, Lipo-C).

Side Effect Profile

  • Extremely well-tolerated; adverse events rare.
  • Mild injection-site stinging possible (cyanocobalamin is slightly acidic).
  • Rare: acneiform skin eruption with high-dose ongoing therapy.
  • Very rare hypersensitivity to cobalt component.
  • Cyanocobalamin releases small amount of cyanide — not clinically significant at therapeutic doses.

Storage Instructions

StateTemperatureDuration
Lyophilized−20°C (−4°F)Up to 24 months
Reconstituted2–8°CUp to 30 days — protect from light
⚠ Research Use Only: Injectable B12 is generally considered safe and nutritional rather than investigational. Consult a healthcare professional for deficiency diagnosis and appropriate dosing.

References

1
Stabler SP. 'Vitamin B12 Deficiency' — NEJM, 2013 View source ↗
2
Carmel R. 'How I treat cobalamin (vitamin B12) deficiency' — Blood, 2008 View source ↗