Sermorelin (10 mg Vial) Dosage Protocol
Sermorelin is a 29-amino-acid synthetic peptide corresponding to the active fragment of native GHRH. Approved historically as a diagnostic for GH deficiency and used in research for GH-axis stimulation.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Once daily (pre-bed) | Half-life: ~10 minutes (effects on GH last hours)
Standard Approach (3 mL = 3.33 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 3.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Weeks 1–2 (titration) | 200 mcg | 6 units | 0.060 mL | 50 doses |
| Weeks 3–4 | 300 mcg | 9 units | 0.090 mL | 33 doses |
| Weeks 5–6 | 400 mcg | 12 units | 0.12 mL | 25 doses |
| Weeks 7–8 (target) | 500 mcg | 15 units | 0.15 mL | 20 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 14 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 400 mcg per dose, once daily (pre-bed) (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Sermorelin (10 mg) vials | 3 vials | 4 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 2 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Sermorelin is the original GHRH analog, with a shorter half-life than CJC-1295 (~10 min vs ~30 min) producing a discrete pulse. It was historically used as a diagnostic for GH deficiency and has the longest published clinical track record of any GHRH analog.
Pre-bed dosing is the most-cited protocol, aligning with the natural overnight GH pulse. Once-daily dosing fits well into a long-term protocol structure.
Dosing Protocol
Daily pre-bed dosing:
- Light (100 mcg pre-bed): Conservative dose, suitable for long-term continuous use.
- Standard (200 mcg pre-bed): Most-cited research dose.
- Higher (300 mcg pre-bed): Some performance/body-composition research uses this.
Cycle structure: 12–24 weeks continuous, then 4-week break. Sermorelin's pulsatile pattern preserves natural feedback better than continuous-elevation alternatives.
Stacking: Sometimes combined with ipamorelin for the GHRH+GHRP synergy, though CJC-1295 no-DAC is more common in stacks.
Timing: Within 1 hour of sleep, fasted (no food/protein in last 2h).
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 14 days, protect from light |
Important Notes
How This Works
Sermorelin is a 29-amino-acid analog of the active N-terminal portion of native GHRH. It binds pituitary GHRH receptors, stimulating endogenous pulsatile GH release. Half-life is shorter than CJC-1295 (~10 min vs ~30 min) producing a brief discrete pulse.
Potential Benefits & Side Effects
Potential Benefits
- Stimulates endogenous GH release via natural pathway.
- Pulsatile rather than continuous — preserves natural feedback.
- Sleep-quality improvements anecdotally reported.
- Body-composition effects via sustained protocols.
Side Effect Profile
- Injection-site reactions.
- Mild flushing post-dose.
- Transient water retention.
- Headache occasionally reported.
Lifestyle Factors
- Pre-bed timing aligns with natural GH pulse during slow-wave sleep.
- Avoid late-evening high-fat meals.
- Resistance training amplifies anabolic effects.
Injection Technique
- Subcutaneous into abdomen, thigh, or upper arm.
- Rotate sites with each dose.
- 90° angle with short insulin needle.