Sermorelin (10 mg Vial) Dosage Protocol
Sermorelin is a 29-amino-acid synthetic peptide corresponding to the active fragment of native GHRH. Approved historically as a diagnostic for GH deficiency and used in research for GH-axis stimulation.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and nightly dosing
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
| Week | Daily Dose (µg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 200 µg (0.2 mg) | 6 units (0.06 mL) |
| Weeks 3–4 | 300 µg (0.3 mg) | 9 units (0.09 mL) |
| Weeks 5–6 | 400 µg (0.4 mg) | 12 units (0.12 mL) |
| Weeks 7–8 | 500 µg (0.5 mg) | 15 units (0.15 mL) |
Frequency: Inject once daily subcutaneously at bedtime. Bedtime administration is strongly recommended because endogenous GH secretion peaks during sleep. This schedule uses the largest practical dilution (3.0 mL) to keep per‑injection units measurable with standard insulin syringes.
For ≤10‑unit (≤0.10 mL) administrations, consider 30‑ or 50‑unit insulin syringes for improved readability.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; gently swirl to dissolve (do not shake vigorously).
- Label with reconstitution date and refrigerate at 2–8 °C (36–46 °F), protected from light.
- Wipe vial stopper with alcohol before each use; use a new sterile needle and syringe for each injection.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Plan based on an 8–week daily protocol with gradual titration (56 injections total).
| Item | Quantity |
|---|---|
| Peptide Vials (Sermorelin, 10 mg each) | 8 weeks (total ~19.6 mg consumed): 2 vials (20 mg total) 12 weeks (similar daily range): 4 vials Tip: Have 1 extra vial as backup in case of spillage or loss. |
| Insulin Syringes (U‑100) | Per week: 7 syringes (1/day) 8 weeks: 56 syringes (recommend 1 × 100‑count box) 12 weeks: 84 syringes (1 × 100‑count box) Preferred: 0.3–0.5 mL size with 28G–31G needle, 5/16″ to 1/2″ length for subcutaneous use. Consider 30‑ or 50‑unit syringes for low‑dose weeks for improved precision. |
| Bacteriostatic Water (10 mL bottles) | 8 weeks (2 vials): 6 mL → 1 × 10 mL bottle 12 weeks (4 vials): 12 mL → 2 × 10 mL bottles Contains benzyl alcohol preservative; do not use if allergic. |
| Alcohol Swabs | Per week: 14 swabs (2/day) 8 weeks: 112 swabs → recommend 2 × 100‑count boxes 12 weeks: 168 swabs → recommend 2 × 100‑count boxes |
Sharps Container: One puncture‑proof disposal container for used needles (sufficient for 56+ syringes).
Protocol Overview
It was historically used as a diagnostic for GH deficiency and has the longest published clinical track record of any GHRH analog. Pre-bed dosing is the most-cited protocol, aligning with the natural overnight GH pulse. Once-daily dosing fits well into a long-term protocol structure.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 14 days, protect from light |
Important Notes
How This Works
Sermorelin is a 29-amino-acid analog of the active N-terminal portion of native GHRH. It binds pituitary GHRH receptors, stimulating endogenous pulsatile GH release. Half-life is shorter than CJC-1295 (~10 min vs ~30 min) producing a brief discrete pulse.
Potential Benefits & Side Effects
Potential Benefits
- Stimulates endogenous GH release via natural pathway.
- Pulsatile rather than continuous — preserves natural feedback.
- Sleep-quality improvements anecdotally reported.
- Body-composition effects via sustained protocols.
Side Effect Profile
- Injection-site reactions.
- Mild flushing post-dose.
- Transient water retention.
- Headache occasionally reported.
Lifestyle Factors
- Pre-bed timing aligns with natural GH pulse during slow-wave sleep.
- Avoid late-evening high-fat meals.
- Resistance training amplifies anabolic effects.
Injection Technique
- Subcutaneous into abdomen, thigh, or upper arm.
- Rotate sites with each dose.
- 90° angle with short insulin needle.