Pinealon (20 mg Vial) Dosage Protocol
Pinealon is a synthetic tetrapeptide (Glu-Asp-Arg) studied by Russian researchers as a brain-targeted bioregulator with neuroprotective and cognitive effects.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Once daily during cycle (10–20 days, repeat every 2–3 months) | Half-life: Short (minutes); cumulative effect
Standard Approach (3 mL = 6.67 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 6.67 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Days 1–5 (titration) | 1 mg | 15 units | 0.15 mL | 20 doses |
| Days 6–14 | 1.5 mg | 22.5 units | 0.22 mL | 13 doses |
| Days 15–20 (target) | 2 mg | 30 units | 0.30 mL | 10 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 30 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 1.5 mg per dose, once daily during cycle (10–20 days, repeat every 2–3 months) (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Pinealon (20 mg) vials | 5 vials | 7 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 2 × 10 mL | 3 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Pinealon is a Russian peptide bioregulator dosed in 10–20 day cycles 1–2× per year, similar to other Khavinson-class peptides. The hypothesized gene-expression-modulation mechanism produces effects that persist for months after the cycle ends.
Dosing Protocol
Cycle dosing (subcutaneous, once daily):
- Days 1–5 (titration): 1.0 mg/day.
- Days 6–14: 1.5 mg/day.
- Days 15–20 (target): 2.0 mg/day.
Cycle structure: 10–20 day active phase, 2–3 month break between cycles. Typically 1–2 cycles per year.
Timing: Once daily during cycle, time of day flexible.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
Pinealon is a synthetic tripeptide bioregulator (Glu-Asp-Arg) developed by Khavinson's group. Like Epitalon, it is hypothesized to act via gene-expression modulation in target tissues — in this case brain cortex and hippocampus. Animal models show neuroprotective effects and improved cognitive performance after stress.
Potential Benefits & Side Effects
Potential Benefits
- Neuroprotective effects in animal stress models.
- Improvements in memory and learning in rodent studies.
- Antioxidant effects on neural tissue.
- Used in Russian protocols for age-related cognitive decline.
Side Effect Profile
- Generally well-tolerated.
- Mild injection-site reactions.
- Long-term human safety data outside Russian literature is limited.
Lifestyle Factors
- Cycle-based dosing — typical 10–20 day cycle, 1–2× per year.
- Pair with cognitive engagement (reading, learning) for compounding effect.
- Adequate sleep amplifies neuroprotective effects.
Injection Technique
- Subcutaneous, rotate sites during cycle.