Kisspeptin (10 mg Vial) Dosage Protocol
Kisspeptin is a hypothalamic neuropeptide encoded by the KISS1 gene, controlling GnRH neurons. It is the master regulator of puberty onset and reproductive function.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Once daily | Half-life: ~30 minutes
Standard Approach (3 mL = 3.33 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 3.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Weeks 1–2 (titration) | 100 mcg | 3 units | 0.030 mL | 100 doses |
| Weeks 3–12 (target) | 200 mcg | 6 units | 0.060 mL | 50 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 200 mcg per dose, once daily (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Kisspeptin (10 mg) vials | 2 vials | 2 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 1 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Kisspeptin sits at the most upstream point of the HPG axis — even higher than GnRH, which it controls. Research applications include functional hypothalamic amenorrhea and as an alternative pathway for axis stimulation in protocols where chronic GnRH would cause receptor desensitization.
Dosing Protocol
Twice-to-three-times weekly dosing:
- Light (50 mcg): Conservative starting dose.
- Standard (100 mcg, 2–3× weekly): Common research dose.
Cycle structure: 4–8 weeks during active research protocol, then reassessment.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
Kisspeptin (the active form is kisspeptin-10) binds the KISS1R receptor on hypothalamic GnRH neurons, triggering GnRH release. This sits upstream of the entire HPG axis and is the gatekeeper of puberty and reproduction.
Research applications include functional hypothalamic amenorrhea, hypogonadotropic hypogonadism, and as an alternative pathway for axis stimulation that bypasses pituitary feedback.
Potential Benefits & Side Effects
Potential Benefits
- Master regulator of HPG axis — most upstream point of stimulation.
- Studied in functional hypothalamic conditions.
- Bypasses pituitary feedback inhibition seen with chronic GnRH.
Side Effect Profile
- Injection-site reactions.
- Mild flushing or headache.
- Short half-life requires frequent dosing.
Lifestyle Factors
- Pair with bloodwork (LH, FSH, T) periodically.
- Adequate caloric intake supports endogenous kisspeptin function.
Injection Technique
- Subcutaneous, rotate sites.