Humanin (10 mg Vial) Dosage Protocol
Humanin is a 24-amino-acid peptide encoded in mitochondrial DNA (16S rRNA), studied for cytoprotective and metabolic effects. Levels decline with age.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Daily during cycle | Half-life: ~30 minutes
Standard Approach (3 mL = 3.33 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 3.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Standard | 5 mg | 150 units | 1.50 mL | 2 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 30 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 5 mg per dose, daily during cycle (7 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Humanin (10 mg) vials | 28 vials | 42 vials |
| Insulin syringes (U-100) | 56 | 84 |
| Bacteriostatic water (10 mL) | 9 × 10 mL | 13 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Humanin protocols are continuous-use rather than cycled given the cytoprotective rather than receptor-stimulating mechanism. Most research uses 8–12 week cycles for assessment, though continuous long-term use is reasonable.
Dosing Protocol
Daily dosing:
- Standard (5 mg/day): Daily research dose.
Cycle: 8–12 weeks active, then reassessment.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
Humanin is a 24-amino-acid peptide encoded within the mitochondrial 16S rRNA gene. It is one of the few mitochondrially encoded peptides with broad signaling activity. Mechanisms include anti-apoptotic effects via Bax inhibition, IGF-binding-protein-3 modulation, and protection against amyloid-beta toxicity in neuronal models.
Potential Benefits & Side Effects
Potential Benefits
- Cytoprotective effects in multiple cell types.
- Neuroprotection in Alzheimer’s-related amyloid models.
- Cardioprotection in ischemia models.
- Improved insulin sensitivity in some studies.
Side Effect Profile
- Generally well-tolerated in available research.
- Mild injection-site reactions possible.
Lifestyle Factors
- Pair with exercise — exercise upregulates endogenous humanin.
- Sleep adequacy supports mitochondrial recovery.
Injection Technique
- Subcutaneous, rotate sites.