Humanin (10 mg Vial) Dosage Protocol
Humanin is a 24-amino-acid peptide encoded in mitochondrial DNA (16S rRNA), studied for cytoprotective and metabolic effects. Levels decline with age.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Community-Reported Use Calculations (3.0 mL = 3.333 mg/mL)
| Research Period | Amount per Administration | U-100 Units (mL) |
|---|---|---|
| 8 weeks | 1 mg | 30 units (0.30 mL) |
| 8 weeks | 2 mg | 60 units (0.60 mL) |
| 8 weeks | 3 mg | 90 units (0.90 mL) |
Frequency: Twice weekly in the recurring community descriptions selected for this worked example. Each row is independent, not a titration schedule.
Research reference only: The 1–3 mg, twice-weekly, eight-week patterns are nonclinical community descriptions. Reports vary materially, and no academic humanin trial establishes these schedule numbers. The academic references support identity, mechanism, model findings, and evidence limits—not the charted regimen.
Reconstitution Steps
- Clean the vial stopper and diluent stopper with alcohol; allow both to dry.
- Draw exactly 3.0 mL of bacteriostatic water with a new sterile syringe, then inject it slowly down the inside wall of the vial.
- Gently swirl or roll until dissolved. Do not shake.
- Label the vial with the compound, concentration, and reconstitution date. Refrigerate at 2–8 °C (36–46 °F) and protect from light.
Storage note: No compound-specific post-reconstitution stability or discard period has been established for this worked research-vial preparation. Follow the lot-specific product documentation for any shorter limit.
This page is for research calculation and educational reference only. It is not medical advice or a direction for human use.
Supplies Needed
Choose one eight-week calculation row. Do not add the rows together.
| Item | Quantity |
|---|---|
| Peptide Vials (Humanin, 10 mg each) | 8 weeks at 1 mg twice weekly: 2 vials 8 weeks at 2 mg twice weekly: 4 vials 8 weeks at 3 mg twice weekly: 5 vials |
| Insulin Syringes (U-100) | 16 calculated administrations: 16 syringes |
| Bacteriostatic Water (10 mL bottles) | 2-vial calculation: 6 mL 4-vial calculation: 12 mL 5-vial calculation: 15 mL |
| Alcohol Swabs | 16 calculated administrations at 2 swabs each: 32 swabs |
Sharps safety: Place used needles and syringes immediately in an approved sharps container.
Protocol Overview
Humanin protocols are continuous-use rather than cycled given the cytoprotective rather than receptor-stimulating mechanism.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 30 days, protect from light |
Important Notes
How This Works
Humanin is a 24-amino-acid peptide encoded within the mitochondrial 16S rRNA gene. It is one of the few mitochondrially encoded peptides with broad signaling activity. Mechanisms include anti-apoptotic effects via Bax inhibition, IGF-binding-protein-3 modulation, and protection against amyloid-beta toxicity in neuronal models.
Potential Benefits & Side Effects
Potential Benefits
- Cytoprotective effects in multiple cell types.
- Neuroprotection in Alzheimer’s-related amyloid models.
- Cardioprotection in ischemia models.
- Improved insulin sensitivity in some studies.
Side Effect Profile
- Generally well-tolerated in available research.
- Mild injection-site reactions possible.
Lifestyle Factors
- Pair with exercise — exercise upregulates endogenous humanin.
- Sleep adequacy supports mitochondrial recovery.
Injection Technique
- Subcutaneous, rotate sites.