Gonadorelin (GnRH) (10 mg Vial) Dosage Protocol
Gonadorelin is synthetic gonadotropin-releasing hormone (GnRH), a 10-amino-acid hypothalamic peptide that stimulates pituitary LH and FSH release.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: 2–3× weekly (Mon/Wed/Fri pattern) | Half-life: ~5 minutes
Standard Approach (10 mL = 1.00 mg/mL)
Reconstituting with 10 mL bacteriostatic water produces a concentration of 1.00 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Weeks 1–2 (initiation) | 50 mcg | 5 units | 0.050 mL | 200 doses |
| Weeks 3–4 (titration) | 100 mcg | 10 units | 0.10 mL | 100 doses |
| Weeks 5–8 (maintenance) | 125 mcg | 12.5 units | 0.12 mL | 80 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 10 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 125 mcg per dose, 2–3× weekly (mon/wed/fri pattern) (3 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| Gonadorelin (GnRH) (10 mg) vials | 1 vials | 1 vials |
| Insulin syringes (U-100) | 24 | 36 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 1 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Gonadorelin (synthetic GnRH) stimulates the entire HPG axis upstream of HCG — preserving pituitary function rather than just testicular Leydig cells. This makes it conceptually appealing as a TRT adjunct, though the short half-life (~5 minutes) makes pulsatile dosing impractical without a pump.
Most research-grade protocols use bolus dosing 2–3× weekly, accepting the non-pulsatile pattern as a tradeoff for practicality.
Dosing Protocol
Two protocol patterns:
- Pulsatile (25 mcg every 90 minutes via pump): Most physiological. Rare in research-grade practice due to pump requirement.
- Sustained (100 mcg, 2–3× weekly): Practical bolus dosing. Most-cited research application.
Cycle structure: Continuous use as TRT adjunct. Bloodwork every 8–12 weeks (LH, FSH, T).
Timing: Alternate days throughout the week (Mon/Wed/Fri pattern typical for 3×/week).
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
Gonadorelin is the active form of native GnRH, a 10-amino-acid hypothalamic decapeptide that stimulates the anterior pituitary to release LH and FSH. The pituitary GnRH receptor requires pulsatile stimulation — continuous high-dose exposure produces receptor desensitization, which is the basis for GnRH agonist use as androgen deprivation in prostate cancer.
For testicular function maintenance during TRT (alternative to HCG), gonadorelin is dosed in pulsatile patterns mimicking natural hypothalamic release, preserving the pituitary-gonadal axis upstream of LH/FSH.
Potential Benefits & Side Effects
Potential Benefits
- Preserves entire HPG axis (vs HCG which acts only at the gonadal level).
- Useful as TRT adjunct for axis preservation.
- Used in fertility restoration protocols.
Side Effect Profile
- Injection-site reactions.
- Continuous (non-pulsatile) high-dose use causes desensitization.
- Mild headache or flushing post-dose.
Lifestyle Factors
- Pulsatile dosing (every 90 min) is more physiological but impractical without pump.
- Most research-grade protocols use 2–3× weekly bolus dosing.
- Pair with bloodwork (LH, FSH, T) every 8–12 weeks.
Injection Technique
- Subcutaneous, rotate sites.
- 90° angle with short insulin needle.