FST 344 (Follistatin) (1 mg Vial) Dosage Protocol
Follistatin (FST 344) is a glycoprotein that inhibits myostatin and activin, studied for muscle growth and tissue repair.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: 2–3× weekly | Half-life: ~12 hours
Standard Approach (3 mL = 0.33 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 0.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Standard | 100 mcg | 30.0 units | 0.30 mL | 10 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 14 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 100 mcg per dose, 2–3× weekly (3 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| FST 344 (Follistatin) (1 mg) vials | 3 vials | 4 vials |
| Insulin syringes (U-100) | 24 | 36 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 2 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
Follistatin (FST 344) suppresses myostatin, removing the natural brake on skeletal muscle hypertrophy. Effects compound during active training cycles — solo dosing without resistance training produces minimal benefit.
WADA prohibited list. Long-term safety data is limited.
Dosing Protocol
Twice-to-three-times-weekly dosing:
- Standard (100 mcg, 2–3×/week): Most-cited research dose.
Cycle structure: 6–8 weeks active training cycle, then 8–12 week break. Conservative cycling given limited long-term data.
Timing: Within 1 hour post-training, sub-Q near target muscle group.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 14 days, protect from light |
Important Notes
How This Works
Follistatin is a glycoprotein that binds and neutralizes myostatin (a muscle growth inhibitor) and activin. By suppressing myostatin, follistatin removes the brake on satellite cell activation and skeletal muscle hypertrophy. Knockout and overexpression studies show dramatic muscle phenotype changes.
Potential Benefits & Side Effects
Potential Benefits
- Myostatin neutralization supports muscle hypertrophy.
- Studied in muscle-wasting conditions.
- Animal models show significant lean mass gains.
Side Effect Profile
- Limited human safety data.
- Theoretical cardiac concerns at very high systemic doses.
Lifestyle Factors
- Pair with resistance training.
- Adequate protein intake.
Injection Technique
- Subcutaneous, rotate sites.