CJC-1295 without DAC (Mod GRF 1-29) (10 mg Vial) Dosage Protocol
CJC-1295 without DAC (also called Modified GRF 1-29) is a 30-amino-acid synthetic GHRH analog with a half-life of ~30 minutes. It produces a discrete GH pulse rather than sustained elevation.
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Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: 1–3× daily | Half-life: ~30 minutes
Standard Approach (3 mL = 3.33 mg/mL)
Reconstituting with 3 mL bacteriostatic water produces a concentration of 3.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Weeks 1–2 (titration) | 100 mcg | 3 units | 0.030 mL | 100 doses |
| Weeks 3–4 | 150 mcg | 4.5 units | 0.045 mL | 66 doses |
| Weeks 5–6 | 200 mcg | 6 units | 0.060 mL | 50 doses |
| Weeks 7–12 (target) | 250 mcg | 7.5 units | 0.075 mL | 40 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 200 mcg per dose, 1–3× daily (3 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| CJC-1295 without DAC (Mod GRF 1-29) (10 mg) vials | 1 vials | 1 vials |
| Insulin syringes (U-100) | 24 | 36 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 1 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
The non-DAC version (Mod GRF 1-29) has a half-life of ~30 minutes — producing a discrete GH pulse rather than sustained elevation. This makes it more physiological than the DAC version but requires more frequent dosing (1–3× daily).
Almost always combined with a GHRP for the synergistic GH-pulse effect — see CJC-1295 + Ipamorelin for the most popular combination.
Dosing Protocol
Daily dosing options:
- Light (100 mcg, once daily pre-bed): Single nightly pulse aligned with overnight GH window.
- Standard (100 mcg, 2–3× daily): Multiple GH pulses across the day. Most-cited research protocol.
- Higher (200 mcg, pre-workout + pre-bed): Heavier protocol pairing pre-training pulse with overnight pulse.
Cycle structure: 8–12 weeks continuous, then 2–4 week break. Pulsatile pattern preserves natural feedback better than DAC version.
Stacking with GHRP: Almost always combined with ipamorelin or another GHRP — the GHRH+GHRP synergy produces 5–10× larger GH pulses than either alone.
Timing: Avoid eating for 30+ minutes after each dose — meals blunt GH release. Pre-bed dosing aligns with natural overnight pulse during slow-wave sleep.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
CJC-1295 without DAC contains the same four stabilizing amino acid substitutions as the DAC version (D-Ala2, Gln8, Ala15, Leu27) but lacks the albumin-binding linker. The result is a stable, short-acting GHRH analog producing a discrete GH pulse over ~30 minutes.
Common practice combines CJC-1295 with ipamorelin (a GHRP) — the GHRH stimulates GH release while the GHRP synergistically amplifies it, mimicking the body’s natural feedback patterns.
Potential Benefits & Side Effects
Potential Benefits
- Discrete pulsatile GH release mimicking physiological pattern.
- Often combined with ipamorelin for synergistic GH pulses.
- Improvements in sleep quality reported.
- Body composition and recovery effects via sustained protocols.
Side Effect Profile
- Injection-site reactions.
- Mild head flushing or fullness post-dose.
- Transient water retention.
- Hunger increase (some users).
Lifestyle Factors
- Avoid eating for 30+ min after injection — meals blunt GH release.
- Pre-bed dosing aligns with natural GH pulse during slow-wave sleep.
- Resistance training amplifies anabolic effects.
Injection Technique
- Subcutaneous into abdomen, thigh, or upper arm.
- Rotate sites with each dose.
- 90° angle with short insulin needle.