Research use only.  Educational reference. Not medical advice. Not for human consumption.
⚖️ Amylin Analog

Cagrilintide (10 mg Vial) Dosage Protocol

Cagrilintide is a long-acting amylin analog studied for weight management, often combined with semaglutide in Phase 2 (CagriSema) trials reporting 15%+ weight loss.

⚡ Quickstart Highlights

Vial size
10 mg
Reconstitution
3 mL BAC water → 3.33 mg/mL
1 U-100 unit =
33.3 mcg
Frequency
Once weekly

Dosing & Reconstitution Guide

Route: Subcutaneous  |  Frequency: Once weekly  |  Half-life: ~7 days

Standard Approach (3 mL = 3.33 mg/mL)

Reconstituting with 3 mL bacteriostatic water produces a concentration of 3.33 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.

Phase / ProtocolDoseU-100 UnitsVolumeDoses per vial
Weeks 1–2 (titration)600 mcg18 units0.18 mL16 doses
Weeks 3–41.2 mg36 units0.36 mL8 doses
Weeks 5–62.4 mg72 units0.72 mL4 doses
Weeks 7–16+ (maintenance)4.5 mg135 units1.35 mL2 doses

Reconstitution Steps

  1. Wipe the vial stopper and BAC water vial with alcohol; let dry.
  2. Draw 3 mL of bacteriostatic water into a sterile syringe.
  3. Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
  4. Gently swirl until fully dissolved. Do not shake.
  5. Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.

Supplies Needed

Estimates for an 8-week and 12-week cycle at 4.5 mg per dose, once weekly (1 dose/week).

Item8-Week Cycle12-Week Cycle
Cagrilintide (10 mg) vials4 vials6 vials
Insulin syringes (U-100)812
Bacteriostatic water (10 mL)2 × 10 mL2 × 10 mL
Alcohol swabs1 × 100-pack2 × 100-pack

Protocol Overview

Cagrilintide is most commonly studied as part of the CagriSema combination, but monotherapy protocols also exist. The amylin-analog mechanism complements GLP-1 agonists rather than replacing them — solo cagrilintide produces ~10% weight loss vs ~15%+ for the combination.

Trial protocols use a 4-week-per-step titration similar to GLP-1 titration. Once-weekly dosing fits alongside the GLP-1 schedule when used in combination.

Cycle length: Phase 2 trials ran 26 weeks; Phase 3 ongoing. Long-term protocols are not yet established.

Dosing Protocol

Standard titration mirrors the GLP-1 class:

Combination dosing: When stacked with semaglutide (CagriSema), both peptides are titrated together on the same weekly schedule.

Half-life: ~7 days, supports flexible day-of-week timing.

Storage Instructions

StateTemperatureDuration
Lyophilized−20°C (−4°F)Up to 24 months, dry & dark
Reconstituted2–8°C (35–46°F)Up to 28 days, protect from light

Important Notes

⚠ Research Use Only: Cagrilintide is investigational, currently in Phase 3 trials. Use is research-grade only.

How This Works

Cagrilintide is a long-acting analog of human amylin — a hormone co-secreted with insulin from pancreatic beta cells. Native amylin slows gastric emptying, suppresses glucagon secretion, and enhances satiety, but has a half-life of minutes. Cagrilintide is fatty-acid acylated for albumin binding, giving once-weekly dosing.

It binds amylin receptors in the hindbrain area postrema, producing satiety and reducing food intake through a mechanism complementary to GLP-1.

Potential Benefits & Side Effects

Potential Benefits

Side Effect Profile

Lifestyle Factors

Injection Technique

References

1
Lau DCW et al. 'Once-weekly cagrilintide for weight management' — Lancet, 2021 View source ↗
2
Frias JP et al. 'CagriSema for type 2 diabetes' — Lancet, 2023 View source ↗