5-Amino-1MQ (50 mg Vial) Dosage Protocol
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), studied for body composition and metabolic effects in adipose tissue.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Subcutaneous Protocol (4 mL = 12.5 mg/mL)
| Phase | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Days 1–2 (Tolerance) | 2.5 mg once daily | 20 units (0.20 mL) |
| Days 3+ (Standard) | 5 mg once daily | 40 units (0.40 mL) |
| Alternative BID | 2.5 mg twice daily | 20 units (0.20 mL) × 2 |
Frequency: Inject once or twice daily subcutaneously. Due to the compound’s plasma half-life of approximately 3.8–6.9 hours, twice-daily (BID) dosing may provide more sustained NNMT inhibition. A single 50 mg vial provides 10–20 days of research material at these doses.
Reconstitution Steps
- Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
- Draw 4.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved—solution should be clear (do not shake). Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; follow the exact formulation’s validated use-by period.
Supplies Needed
Plan based on research duration using the 50 mg vial format at 5 mg/day.
| Item | Quantity |
|---|---|
| 5-Amino-1MQ Vials (50 mg each) | 1 week (5 mg/day): approximately 1 vial required 2 weeks (5 mg/day): approximately 1 vial required 4 weeks (5 mg/day): approximately 2 vials required |
| Insulin Syringes (U-100) | Per week (once daily): 7 syringes Per week (BID): 14 syringes 4 weeks (once daily): 28 syringes |
| Bacteriostatic Water (10 mL bottles) | 1 week (1 vial): 4 mL — 1 bottle required (10 mL each) 2 weeks (1 vial): 4 mL — 1 bottle required (10 mL each) 4 weeks (2 vials): 8 mL — 1 bottle required (10 mL each) |
| Alcohol Swabs | Per week (once daily): 14 swabs 4 weeks (once daily): 56 swabs — 1 box required (100 swabs each) |
Protocol Overview
Most published research uses oral dosing, but subcutaneous protocols exist for higher-bioavailability use.
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
5-Amino-1MQ inhibits nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in obese adipose tissue that consumes SAM (S-adenosyl methionine) and NAD+. NNMT inhibition restores SAM and NAD+ pools, supporting energy metabolism and reducing adipocyte expansion.
Potential Benefits & Side Effects
Potential Benefits
- Reduced adipose tissue mass in rodent models.
- Improved insulin sensitivity and glucose handling.
- Restoration of NAD+ and SAM levels in obese tissue.
- Synergy with caloric restriction and exercise.
Side Effect Profile
- Generally well-tolerated in available research.
- Long-term human safety data is limited.
Lifestyle Factors
- Pair with caloric deficit and exercise for fat-loss outcomes.
- Adequate methionine and B-vitamin intake supports the SAM cycle.
Injection Technique
- Most commonly oral; some research protocols use subcutaneous.
- For sub-Q: room-temp solution, 90° angle.