5-Amino-1MQ (50 mg Vial) Dosage Protocol
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), studied for body composition and metabolic effects in adipose tissue.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Once or twice daily (subcutaneous) | Half-life: ~3.8–6.9 hours
Standard Approach (4 mL = 12.50 mg/mL)
Reconstituting with 4 mL bacteriostatic water produces a concentration of 12.50 mg/mL. Volume per dose changes with concentration; mg dose itself does not change between vial sizes.
| Phase / Protocol | Dose | U-100 Units | Volume | Doses per vial |
|---|---|---|---|---|
| Days 1–2 (tolerance) | 2.5 mg | 20 units | 0.20 mL | 20 doses |
| Days 3+ (target) | 5 mg | 40 units | 0.40 mL | 10 doses |
Reconstitution Steps
- Wipe the vial stopper and BAC water vial with alcohol; let dry.
- Draw 4 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the peptide vial. Do not aim at the powder.
- Gently swirl until fully dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Needed
Estimates for an 8-week and 12-week cycle at 5 mg per dose, once or twice daily (subcutaneous) (14 doses/week).
| Item | 8-Week Cycle | 12-Week Cycle |
|---|---|---|
| 5-Amino-1MQ (50 mg) vials | 12 vials | 17 vials |
| Insulin syringes (U-100) | 112 | 168 |
| Bacteriostatic water (10 mL) | 5 × 10 mL | 7 × 10 mL |
| Alcohol swabs | 1 × 100-pack | 2 × 100-pack |
Protocol Overview
5-Amino-1MQ is unusual among compounds on this site in that it is a small molecule rather than a peptide, and oral bioavailability is preserved (capsule formulations exist alongside research-grade injectable). Most published research uses oral dosing, but subcutaneous protocols exist for higher-bioavailability use.
Effects on body composition are gradual — visible changes typically appear after 8–12 weeks of consistent use paired with caloric deficit.
Dosing Protocol
Daily dosing schedule (subcutaneous):
- Tolerance (2.5 mg/day): Days 1–2 to assess response.
- Standard (5 mg/day): Days 3+ target maintenance dose; once daily.
- BID alternative (2.5 mg twice daily): Half-life is 3.8–6.9 hours, so split AM/PM dosing may provide more sustained NNMT inhibition.
Cycle structure: 8–12 weeks daily, then 4-week break.
Timing: Morning dosing typical to align with natural cortisol/glucose rhythm. BID protocol splits AM/PM (early morning + late afternoon).
Storage Instructions
| State | Temperature | Duration |
|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months, dry & dark |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days, protect from light |
Important Notes
How This Works
5-Amino-1MQ inhibits nicotinamide N-methyltransferase (NNMT), an enzyme overexpressed in obese adipose tissue that consumes SAM (S-adenosyl methionine) and NAD+. NNMT inhibition restores SAM and NAD+ pools, supporting energy metabolism and reducing adipocyte expansion.
Potential Benefits & Side Effects
Potential Benefits
- Reduced adipose tissue mass in rodent models.
- Improved insulin sensitivity and glucose handling.
- Restoration of NAD+ and SAM levels in obese tissue.
- Synergy with caloric restriction and exercise.
Side Effect Profile
- Generally well-tolerated in available research.
- Long-term human safety data is limited.
Lifestyle Factors
- Pair with caloric deficit and exercise for fat-loss outcomes.
- Adequate methionine and B-vitamin intake supports the SAM cycle.
Injection Technique
- Most commonly oral; some research protocols use subcutaneous.
- For sub-Q: room-temp solution, 90° angle.